R-02-04 A Biosensor Platform for Detection of Fish Pathogens

Maine Sea Grant Home


 

Paul Millard
Laboratory for Advanced Surface Science and Technology
Sawyer Research Center
University of Maine
Orono, ME 04469
207.581.2265
paul.millard@maine.edu

Carol Kim
Dept. of Biochemistry, Microbiology and Molecular Biology
185 Hitchner Hall
University of Maine
Orono, ME 04469
207.581.2803
carolkim@maine.edu

John Vetelino
Electrical and Computer Engineering
113 Barrows Hall
University of Maine
Orono, ME 04469
207.581.2264
vet@eece.maine.edu

Corinne Pierce
Dept. of Biochemistry, Microbiology and Molecular Biology
158 Hitchner Hall
Orono, ME 04469
207.581.2820
cpierce@maine.edu

Early detection of microbial pathogens could greatly reduce the substantial economic losses to the aquaculture and fish farming industries. However, conventional approaches to direct microbial sampling of marine organisms are typically slow and labor-intensive. This study will combine robust molecular and solid-state electronic technologies to develop an inexpensive hybrid biosensor that can be used by individuals without specialized training to detect microbial pathogens quickly, accurately, and reliably.

2 year project, 2001-2003
Year 1: $70,000
Year 2: $70,000
Total: $140,000

 

Associated Publications

McCarthy, E.L., T.J. Egeler, L.E. Bickerstaff, M.P. da Cunha and P.J. Millard, 2005, Rapid Detection of IHNV by Molecular Padlock Recognition and Surface-Associated Isothermal Amplification, SPIE Proceedings - Optics East 2005: Sensors and Applications.

Hu, Y., L.A. French, K. Radecsky, M.P. da Cunha, P. Millard, and J. Vetelino, 2004, A Lateral Field Excited Liquid Acoustic Wave Sensor, IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control, 51:1373-1380.