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Step by Step Overview of Microbial Source Tracking
Step 1: Collect water samples from streams and
estuaries.
Step 2: Membrane filtration of water samples
for fecal coliform and E. coli.
Step 3: Isolation of E. coli from
certain samples.
Step 4: Collect fecal samples.
Step 5: Serial dilution and membrane filtration
of fecal samples.
Step 6: Map the results, select which isolates
to ribotype.
Step 7: Ribotyping of isolates (contact UNH Jackson
Estuarine Lab for more info).
Step 8: Match the unknown-source stream/estuary
E. coli to known-source E. coli from fecal samples.
To learn more or to volunteer, contact us:
Fred Dillon or
Cayce Dalton at
Wells National Estuarine Research Reserve
Phone: 207-646-1555, ext. 103
Fax: 207-646-2930
